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lamb1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology lamb1
    A) A fixed 5-plex panel outlines different cell types in skeletal muscle: M-I fibers (TNNC1, yellow), M-II fibers (MYH2, purple), satellite cells (PAX7, cyan), fibroblasts (FBLN2, red), and endothelial cells (CD34, white). B-C) Multiplex staining of five different DEGs is visualized in green (COL4A3, COL15A1, ELN, <t>LAMB1,</t> and XIRP2), with the top panels showing only the DEG together with DAPI, and bottom panels displaying all seven channels (5-plex panel, DEG, and DAPI). Representative images are shown for control samples, PCOS patients before treatment (PCOS week 0), and PCOS patients after 16 weeks of metformin treatment.
    Lamb1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sc+17810/Laminin+%CE%B2-1+Antibody/bio_rxiv__64898__2026__03__13__711523-370-19-21
    Average 93 stars, based on 75 article reviews
    lamb1 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Cell type–resolved transcriptomic map of skeletal muscle in women with polycystic ovary syndrome"

    Article Title: Cell type–resolved transcriptomic map of skeletal muscle in women with polycystic ovary syndrome

    Journal: bioRxiv

    doi: 10.64898/2026.03.13.711523

    A) A fixed 5-plex panel outlines different cell types in skeletal muscle: M-I fibers (TNNC1, yellow), M-II fibers (MYH2, purple), satellite cells (PAX7, cyan), fibroblasts (FBLN2, red), and endothelial cells (CD34, white). B-C) Multiplex staining of five different DEGs is visualized in green (COL4A3, COL15A1, ELN, LAMB1, and XIRP2), with the top panels showing only the DEG together with DAPI, and bottom panels displaying all seven channels (5-plex panel, DEG, and DAPI). Representative images are shown for control samples, PCOS patients before treatment (PCOS week 0), and PCOS patients after 16 weeks of metformin treatment.
    Figure Legend Snippet: A) A fixed 5-plex panel outlines different cell types in skeletal muscle: M-I fibers (TNNC1, yellow), M-II fibers (MYH2, purple), satellite cells (PAX7, cyan), fibroblasts (FBLN2, red), and endothelial cells (CD34, white). B-C) Multiplex staining of five different DEGs is visualized in green (COL4A3, COL15A1, ELN, LAMB1, and XIRP2), with the top panels showing only the DEG together with DAPI, and bottom panels displaying all seven channels (5-plex panel, DEG, and DAPI). Representative images are shown for control samples, PCOS patients before treatment (PCOS week 0), and PCOS patients after 16 weeks of metformin treatment.

    Techniques Used: Multiplex Assay, Staining, Control

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    Incubation:

    Article Title: ADAR1 Isoforms Regulate Let-7d Processing in Idiopathic Pulmonary Fibrosis
    Article Snippet: .. Membranes were incubated overnight with a primary antibody at 4 °C (ADAR1, sc-271854; Lamin B, sc-17810, Santa-Cruz Biotechnology), washed with 0.05% PBS-T, and incubated for 1 h at room temperature with an HRP-linked secondary antibody, which was detected with the Super Signal West Dura Extended Duration Substrate detection solutions (Thermo-Scientific, San Francisco, CA, USA). .. The signal was detected and analyzed with the Chemi-Doc technology system (Bio-Rad, Hercules, CA, USA).

    Article Title: Activation of the NALP3-CASP1-IL-1 β Inflammatory Pathway by Pesticide Exposure in Human Umbilical Vein Endothelial Cells
    Article Snippet: To visualize the protein expression, 10,000 HUVEC cells were grown on sterilized glass coverslips (1943-10012A, Bellco Glass, Vineland, NJ, USA); after the treatment with pesticides of 10 days, the cells were fixed with PLP (periodate–lysine–paraformaldehyde) solution in PB for 30 min at 4 °C. .. After this time, cells were washed 3 times with PB for 5 min and permeabilized with 0.5% Triton X-100 (9474680K, LKB-Produkter AB, Bromma, Sweden) in PB for 10 min; 5% skim milk in PB was added to block nonspecific for 1 h. Primary monoclonal antibodies against human NALP3 (dilution: 1:400) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CASP1 (dilution: 1:400) (sc-17810, Santa Cruz Biotechnology), and IL-1 β (1:400) (sc-32294, Santa Cruz Biotechnology) were diluted in 0.5% non-fat milk solution and the samples were incubated for 2 h in a humidified environment at RT in the dark. .. After 3 washes for 5 min each in PB, Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (1:400, 1851447, Life Technologies) was diluted in 0.5% of blocking solution, performing the incubation in the same conditions as before; simultaneously, rhodamine-phalloidin (1:150, 2892779, Life Technologies) was added for the duration of this incubation.

    Article Title: Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
    Article Snippet: .. The latter were blocked in 5% of non-fat milk in PBS 0.1% Tween-20, then membranes were incubated with primary antibodies anti-human Fibronectin (antibody dilution 1:750) (sc-8422, Santa Cruz Biotechnology), anti-Laminin (antibody dilution 1:750) (sc-17810, Santa Cruz Biotechnology), N-cadherin (antibody dilution 1:750) (sc-59987, Santa Cruz Biotechnology), anti-RUNX2 (antibody dilution 1:500) (sc-390351, Santa Cruz Biotechnology), anti-OPN (antibody dilution 1:350) (sc-21742, Santa Cruz Biotechnology), anti-SPARC (antibody dilution 1:350) (sc-25574, Santa Cruz Biotechnology) and anti-beta-actin (antibody dilution 1:750) (sc-69879, Santa Cruz Biotechnology). .. After five washes in PBS (Lonza) containing 0.1% Tween-20, membranes were incubated for 1 h at room temperature with peroxidase-conjugated secondary antibody anti-mouse (A90-116P Goat anti-mouse, Abcam, Milan, Italy) 1:2000 diluted in 1X PBS (Lonza), 3% milk, and 0.1% Tween-20 [ ].

    Article Title: Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences.
    Article Snippet: .. The latter were blocked in 5% of non-fat milk in PBS 0.1% Tween-20, then membranes were incubated with primary antibodies anti-human Fibronectin (antibody dilution 1:750) (sc-8422, Santa Cruz Biotechnology), anti-Laminin (antibody dilution 1:750) (sc-17810, Santa Cruz Biotechnology), N-cadherin (antibody dilution 1:750) (sc-59987, Santa Cruz Biotechnology), anti-RUNX2 (antibody dilution 1:500) (sc-390351, Santa Cruz Biotechnology), anti-OPN (antibody dilution 1:350) (sc-21742, Santa Cruz Biotechnology), anti-SPARC (antibody dilution 1:350) (sc-25574, Santa Cruz Biotechnology) and anti-beta-actin (antibody dilution 1:750) (sc-69879, Santa Cruz Biotechnology). .. After five washes in PBS (Lonza) containing 0.1% Tween-20, membranes were incubated for 1 h at room temperature with peroxidase-conjugated secondary antibody anti-mouse (A90-116P Goat anti-mouse, Abcam, Milan, Italy) 1:2000 diluted in 1X PBS (Lonza), 3% Biomedicines 2021, 9, 1710 6 of 14 milk, and 0.1% Tween-20 [31].

    Article Title: Activation of the NALP3-CASP1-IL-1 β Inflammatory Pathway by Pesticide Exposure in Human Umbilical Vein Endothelial Cells.
    Article Snippet: To visualize the protein expression, 10,000 HUVEC cells were grown on sterilized glass coverslips (1943-10012A, Bellco Glass, Vineland, NJ, USA); after the treatment with pesticides of 10 days, the cells were fixed with PLP (periodate–lysine–paraformaldehyde) solution in PB for 30 min at 4 ◦C. .. After this time, cells were washed 3 times with PB for 5 min and permeabilized with 0.5% Triton X-100 (9474680K, LKB-Produkter AB, Bromma, Sweden) in PB for 10 min; 5% skim milk in PB was added to block nonspecific for 1 h. Primary monoclonal antibodies against human NALP3 (dilution: 1:400) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CASP1 (dilution: 1:400) (sc-17810, Santa Cruz Biotechnology), and IL-1 β (1:400) (sc-32294, Santa Cruz Biotechnology) were diluted in 0.5% non-fat milk solution and the samples were incubated for 2 h in a humidified environment at RT in the dark. .. After 3 washes for 5 min each in PB, Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (1:400, 1851447, Life Technologies) was diluted in 0.5% of blocking solution, performing the incubation in the same conditions as before; simultaneously, rhodamine-phalloidin (1:150, 2892779, Life Technologies) was added for the duration of this incubation.

    Bioprocessing:

    Article Title: Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
    Article Snippet: .. Primary monoclonal antibodies to anti-human Fibronectin (antibody dilution 1:200) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Laminin (antibody dilution 1:200) (sc-17810, Santa Cruz Biotechnology), N-cadherin (sc-59987, Santa Cruz Biotechnology) and RUNX2 (sc-399351, Santa Cruz Biotechnology) were used, followed by Alexa Fluor 488 green fluorescence conjugated goat anti-mouse as secondary antibodies (cat. n. A 11029, Molecular Probes, Eugene, OR, USA). .. Then, Alexa Fluor 594 phalloidin red fluorescence conjugate (antibody dilution 1:200) (cat. n. A12381, Molecular Probes) was used to highlight the cytoskeleton actin and TOPRO (dye dilution 1:200) (cat. n. T3606, Molecular Probes) was used to stain the cell nuclei [ ].

    Article Title: Activation of the NALP3-CASP1-IL-1 β Inflammatory Pathway by Pesticide Exposure in Human Umbilical Vein Endothelial Cells
    Article Snippet: To visualize the protein expression, 10,000 HUVEC cells were grown on sterilized glass coverslips (1943-10012A, Bellco Glass, Vineland, NJ, USA); after the treatment with pesticides of 10 days, the cells were fixed with PLP (periodate–lysine–paraformaldehyde) solution in PB for 30 min at 4 °C. .. After this time, cells were washed 3 times with PB for 5 min and permeabilized with 0.5% Triton X-100 (9474680K, LKB-Produkter AB, Bromma, Sweden) in PB for 10 min; 5% skim milk in PB was added to block nonspecific for 1 h. Primary monoclonal antibodies against human NALP3 (dilution: 1:400) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CASP1 (dilution: 1:400) (sc-17810, Santa Cruz Biotechnology), and IL-1 β (1:400) (sc-32294, Santa Cruz Biotechnology) were diluted in 0.5% non-fat milk solution and the samples were incubated for 2 h in a humidified environment at RT in the dark. .. After 3 washes for 5 min each in PB, Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (1:400, 1851447, Life Technologies) was diluted in 0.5% of blocking solution, performing the incubation in the same conditions as before; simultaneously, rhodamine-phalloidin (1:150, 2892779, Life Technologies) was added for the duration of this incubation.

    Article Title: Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences.
    Article Snippet: .. Primary monoclonal antibodies to anti-human Fibronectin (antibody dilution 1:200) (sc8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Laminin (antibody dilution 1:200) (sc-17810, Santa Cruz Biotechnology), N-cadherin (sc-59987, Santa Cruz Biotechnology) and RUNX2 (sc-399351, Santa Cruz Biotechnology) were used, followed by Alexa Fluor 488 green fluorescence conjugated goat anti-mouse as secondary antibodies (cat. n. A 11029, Molecular Probes, Eugene, OR, USA). .. Then, Alexa Fluor 594 phalloidin red fluorescence conjugate (antibody dilution 1:200) (cat. n. A12381, Molecular Probes) was used to highlight the cytoskeleton actin and TOPRO (dye dilution 1:200) (cat. n. T3606, Molecular Probes) was used to stain the cell nuclei [29].

    Article Title: Activation of the NALP3-CASP1-IL-1 β Inflammatory Pathway by Pesticide Exposure in Human Umbilical Vein Endothelial Cells.
    Article Snippet: To visualize the protein expression, 10,000 HUVEC cells were grown on sterilized glass coverslips (1943-10012A, Bellco Glass, Vineland, NJ, USA); after the treatment with pesticides of 10 days, the cells were fixed with PLP (periodate–lysine–paraformaldehyde) solution in PB for 30 min at 4 ◦C. .. After this time, cells were washed 3 times with PB for 5 min and permeabilized with 0.5% Triton X-100 (9474680K, LKB-Produkter AB, Bromma, Sweden) in PB for 10 min; 5% skim milk in PB was added to block nonspecific for 1 h. Primary monoclonal antibodies against human NALP3 (dilution: 1:400) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CASP1 (dilution: 1:400) (sc-17810, Santa Cruz Biotechnology), and IL-1 β (1:400) (sc-32294, Santa Cruz Biotechnology) were diluted in 0.5% non-fat milk solution and the samples were incubated for 2 h in a humidified environment at RT in the dark. .. After 3 washes for 5 min each in PB, Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (1:400, 1851447, Life Technologies) was diluted in 0.5% of blocking solution, performing the incubation in the same conditions as before; simultaneously, rhodamine-phalloidin (1:150, 2892779, Life Technologies) was added for the duration of this incubation.

    Fluorescence:

    Article Title: Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
    Article Snippet: .. Primary monoclonal antibodies to anti-human Fibronectin (antibody dilution 1:200) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Laminin (antibody dilution 1:200) (sc-17810, Santa Cruz Biotechnology), N-cadherin (sc-59987, Santa Cruz Biotechnology) and RUNX2 (sc-399351, Santa Cruz Biotechnology) were used, followed by Alexa Fluor 488 green fluorescence conjugated goat anti-mouse as secondary antibodies (cat. n. A 11029, Molecular Probes, Eugene, OR, USA). .. Then, Alexa Fluor 594 phalloidin red fluorescence conjugate (antibody dilution 1:200) (cat. n. A12381, Molecular Probes) was used to highlight the cytoskeleton actin and TOPRO (dye dilution 1:200) (cat. n. T3606, Molecular Probes) was used to stain the cell nuclei [ ].

    Article Title: Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences.
    Article Snippet: .. Primary monoclonal antibodies to anti-human Fibronectin (antibody dilution 1:200) (sc8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Laminin (antibody dilution 1:200) (sc-17810, Santa Cruz Biotechnology), N-cadherin (sc-59987, Santa Cruz Biotechnology) and RUNX2 (sc-399351, Santa Cruz Biotechnology) were used, followed by Alexa Fluor 488 green fluorescence conjugated goat anti-mouse as secondary antibodies (cat. n. A 11029, Molecular Probes, Eugene, OR, USA). .. Then, Alexa Fluor 594 phalloidin red fluorescence conjugate (antibody dilution 1:200) (cat. n. A12381, Molecular Probes) was used to highlight the cytoskeleton actin and TOPRO (dye dilution 1:200) (cat. n. T3606, Molecular Probes) was used to stain the cell nuclei [29].

    Blocking Assay:

    Article Title: Activation of the NALP3-CASP1-IL-1 β Inflammatory Pathway by Pesticide Exposure in Human Umbilical Vein Endothelial Cells
    Article Snippet: To visualize the protein expression, 10,000 HUVEC cells were grown on sterilized glass coverslips (1943-10012A, Bellco Glass, Vineland, NJ, USA); after the treatment with pesticides of 10 days, the cells were fixed with PLP (periodate–lysine–paraformaldehyde) solution in PB for 30 min at 4 °C. .. After this time, cells were washed 3 times with PB for 5 min and permeabilized with 0.5% Triton X-100 (9474680K, LKB-Produkter AB, Bromma, Sweden) in PB for 10 min; 5% skim milk in PB was added to block nonspecific for 1 h. Primary monoclonal antibodies against human NALP3 (dilution: 1:400) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CASP1 (dilution: 1:400) (sc-17810, Santa Cruz Biotechnology), and IL-1 β (1:400) (sc-32294, Santa Cruz Biotechnology) were diluted in 0.5% non-fat milk solution and the samples were incubated for 2 h in a humidified environment at RT in the dark. .. After 3 washes for 5 min each in PB, Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (1:400, 1851447, Life Technologies) was diluted in 0.5% of blocking solution, performing the incubation in the same conditions as before; simultaneously, rhodamine-phalloidin (1:150, 2892779, Life Technologies) was added for the duration of this incubation.

    Article Title: Activation of the NALP3-CASP1-IL-1 β Inflammatory Pathway by Pesticide Exposure in Human Umbilical Vein Endothelial Cells.
    Article Snippet: To visualize the protein expression, 10,000 HUVEC cells were grown on sterilized glass coverslips (1943-10012A, Bellco Glass, Vineland, NJ, USA); after the treatment with pesticides of 10 days, the cells were fixed with PLP (periodate–lysine–paraformaldehyde) solution in PB for 30 min at 4 ◦C. .. After this time, cells were washed 3 times with PB for 5 min and permeabilized with 0.5% Triton X-100 (9474680K, LKB-Produkter AB, Bromma, Sweden) in PB for 10 min; 5% skim milk in PB was added to block nonspecific for 1 h. Primary monoclonal antibodies against human NALP3 (dilution: 1:400) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CASP1 (dilution: 1:400) (sc-17810, Santa Cruz Biotechnology), and IL-1 β (1:400) (sc-32294, Santa Cruz Biotechnology) were diluted in 0.5% non-fat milk solution and the samples were incubated for 2 h in a humidified environment at RT in the dark. .. After 3 washes for 5 min each in PB, Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (1:400, 1851447, Life Technologies) was diluted in 0.5% of blocking solution, performing the incubation in the same conditions as before; simultaneously, rhodamine-phalloidin (1:150, 2892779, Life Technologies) was added for the duration of this incubation.



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    Image Search Results


    A) A fixed 5-plex panel outlines different cell types in skeletal muscle: M-I fibers (TNNC1, yellow), M-II fibers (MYH2, purple), satellite cells (PAX7, cyan), fibroblasts (FBLN2, red), and endothelial cells (CD34, white). B-C) Multiplex staining of five different DEGs is visualized in green (COL4A3, COL15A1, ELN, LAMB1, and XIRP2), with the top panels showing only the DEG together with DAPI, and bottom panels displaying all seven channels (5-plex panel, DEG, and DAPI). Representative images are shown for control samples, PCOS patients before treatment (PCOS week 0), and PCOS patients after 16 weeks of metformin treatment.

    Journal: bioRxiv

    Article Title: Cell type–resolved transcriptomic map of skeletal muscle in women with polycystic ovary syndrome

    doi: 10.64898/2026.03.13.711523

    Figure Lengend Snippet: A) A fixed 5-plex panel outlines different cell types in skeletal muscle: M-I fibers (TNNC1, yellow), M-II fibers (MYH2, purple), satellite cells (PAX7, cyan), fibroblasts (FBLN2, red), and endothelial cells (CD34, white). B-C) Multiplex staining of five different DEGs is visualized in green (COL4A3, COL15A1, ELN, LAMB1, and XIRP2), with the top panels showing only the DEG together with DAPI, and bottom panels displaying all seven channels (5-plex panel, DEG, and DAPI). Representative images are shown for control samples, PCOS patients before treatment (PCOS week 0), and PCOS patients after 16 weeks of metformin treatment.

    Article Snippet: Primary antibodies for the DEGs were: COL4A2 (C1926, Sigma-Aldrich), COL15A1 (HPA017913, Atlas Antibodies AB), ELN (HPA018111, Atlas Antibodies AB), LAMB1 (sc-17763, Santa Cruz Biotechnology, Dallas, TX), and XIRP2 (HPA074599, Atlas Antibodies AB).

    Techniques: Multiplex Assay, Staining, Control

    Figure 4. The bar graph shows the statistical analysis, where data are expressed as mean ± standard deviation (SD) from three independent experiments evidencing mRNA levels of CASP1 between ctrl cells (A) and each single pesticide b (B), py (C), pr (D), lc (E) with the other experimental points. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparison test. Significant differences compared to the control are indicated (** p < 0.01, *** p < 0.001). The experiment was conducted in triplicate.

    Journal: International journal of molecular sciences

    Article Title: Activation of the NALP3-CASP1-IL-1 β Inflammatory Pathway by Pesticide Exposure in Human Umbilical Vein Endothelial Cells.

    doi: 10.3390/ijms26104947

    Figure Lengend Snippet: Figure 4. The bar graph shows the statistical analysis, where data are expressed as mean ± standard deviation (SD) from three independent experiments evidencing mRNA levels of CASP1 between ctrl cells (A) and each single pesticide b (B), py (C), pr (D), lc (E) with the other experimental points. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparison test. Significant differences compared to the control are indicated (** p < 0.01, *** p < 0.001). The experiment was conducted in triplicate.

    Article Snippet: After this time, cells were washed 3 times with PB for 5 min and permeabilized with 0.5% Triton X-100 (9474680K, LKB-Produkter AB, Bromma, Sweden) in PB for 10 min; 5% skim milk in PB was added to block nonspecific for 1 h. Primary monoclonal antibodies against human NALP3 (dilution: 1:400) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CASP1 (dilution: 1:400) (sc-17810, Santa Cruz Biotechnology), and IL-1 β (1:400) (sc-32294, Santa Cruz Biotechnology) were diluted in 0.5% non-fat milk solution and the samples were incubated for 2 h in a humidified environment at RT in the dark.

    Techniques: Standard Deviation, Comparison, Control

    Figure 10. The figures show the expression of CASP1 analyzed by immunofluorescence microscopy per- formed in HUVEC cells cultured alone (A1), with Boscalid (A2), Pyraclostrobin (A3), Propamocarb (A4), Lamba-cyhalothrin (A5), Boscalid + Pyraclostrobin (A6), Propamocarb + Lamba-cyhalothrin (A7), Boscalid + Pyraclostrobin + Propamocarb (A8), and Boscalid + Pyraclostrobin + Lamba-cyhalothrin (A9) over a period of 10 days. The experiment was conducted three times, each independently.

    Journal: International journal of molecular sciences

    Article Title: Activation of the NALP3-CASP1-IL-1 β Inflammatory Pathway by Pesticide Exposure in Human Umbilical Vein Endothelial Cells.

    doi: 10.3390/ijms26104947

    Figure Lengend Snippet: Figure 10. The figures show the expression of CASP1 analyzed by immunofluorescence microscopy per- formed in HUVEC cells cultured alone (A1), with Boscalid (A2), Pyraclostrobin (A3), Propamocarb (A4), Lamba-cyhalothrin (A5), Boscalid + Pyraclostrobin (A6), Propamocarb + Lamba-cyhalothrin (A7), Boscalid + Pyraclostrobin + Propamocarb (A8), and Boscalid + Pyraclostrobin + Lamba-cyhalothrin (A9) over a period of 10 days. The experiment was conducted three times, each independently.

    Article Snippet: After this time, cells were washed 3 times with PB for 5 min and permeabilized with 0.5% Triton X-100 (9474680K, LKB-Produkter AB, Bromma, Sweden) in PB for 10 min; 5% skim milk in PB was added to block nonspecific for 1 h. Primary monoclonal antibodies against human NALP3 (dilution: 1:400) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CASP1 (dilution: 1:400) (sc-17810, Santa Cruz Biotechnology), and IL-1 β (1:400) (sc-32294, Santa Cruz Biotechnology) were diluted in 0.5% non-fat milk solution and the samples were incubated for 2 h in a humidified environment at RT in the dark.

    Techniques: Expressing, Immunofluorescence, Microscopy, Cell Culture

    Figure 13. The bar graph shows the statistical analysis, where data are expressed as mean ± standard deviation (SD) from three independent experiments evidencing the CASP1 fluorescence intensity between ctrl cells (A) and each single pesticide b (B), py (C), pr (D), lc (E) with the other experimental points. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparison test. Significant differences compared to the control are indicated (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Journal: International journal of molecular sciences

    Article Title: Activation of the NALP3-CASP1-IL-1 β Inflammatory Pathway by Pesticide Exposure in Human Umbilical Vein Endothelial Cells.

    doi: 10.3390/ijms26104947

    Figure Lengend Snippet: Figure 13. The bar graph shows the statistical analysis, where data are expressed as mean ± standard deviation (SD) from three independent experiments evidencing the CASP1 fluorescence intensity between ctrl cells (A) and each single pesticide b (B), py (C), pr (D), lc (E) with the other experimental points. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparison test. Significant differences compared to the control are indicated (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Article Snippet: After this time, cells were washed 3 times with PB for 5 min and permeabilized with 0.5% Triton X-100 (9474680K, LKB-Produkter AB, Bromma, Sweden) in PB for 10 min; 5% skim milk in PB was added to block nonspecific for 1 h. Primary monoclonal antibodies against human NALP3 (dilution: 1:400) (sc-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CASP1 (dilution: 1:400) (sc-17810, Santa Cruz Biotechnology), and IL-1 β (1:400) (sc-32294, Santa Cruz Biotechnology) were diluted in 0.5% non-fat milk solution and the samples were incubated for 2 h in a humidified environment at RT in the dark.

    Techniques: Standard Deviation, Fluorescence, Comparison, Control